1 vertor , dh5a competent cells and long template expanded pcr kit were from invitrogen company 1载体, dh5a感受态细菌及pcr扩增试剂购自invitrogen公司。
Ligate the insert hbrp to a pgex - 5x - 1 expression vector . prepare eoli . bl21 competent cells 制备eoli . bl21感受态细胞,将pgex一hb即表达质粒,转化bl一21感受态细胞。
Mineral oils stay at the injection site and are progressively eliminated by competent cells like macrophages 矿物油留在注射处象巨噬细胞这样有反应能力的细胞使得排泄量日益增多。
Pgem - t vector system , jm109 competent cells , reverse transcription kit and in vitro translation kit were purchased from promega company Pgem一t载体,感受态细菌jm109 ,反转录试剂盒和体外翻译试剂盒均购自promega公司。
Effects of blocking in vivo cd80 cd86 costimulatory signal at the early gestation on the costimulatory molecules expression of immuno - competent cells in the abortion - prone model 86协同刺激信号对自然流产模型免疫活性细胞协同刺激分子表达的影响